Hotspot

rps19-rpl23

LSC · 80328-82040 · 1713 bp

Back to Christella

Pi 0.0117

MarkerSeek score 65.00

Primer available yes

Label rps19-rpl23

Feature Metadata

label_name: rps19-rpl23
feature_type: igs
region: LSC
length_bp: 1713
variable_sites: 20
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl23_p1	GACAGGTACGACTGCAGAAG	TGAGTTTGAATTCCGCGAGT	55.0	45.0	58.0	57.8	1877 bp	1.0	79.4
rps19-rpl23_p2	GACAGGTACGACTGCAGAAG	ATGATTGCTAGACTTCGGGC	55.0	50.0	58.0	57.8	1848 bp	1.0	78.8
rps19-rpl23_p3	GCGAGACCAAGTTACAACCA	TGAGTTTGAATTCCGCGAGT	50.0	45.0	58.1	57.8	1856 bp	1.0	78.5
rps19-rpl23_p4	GCGAGACCAAGTTACAACCA	ATGATTGCTAGACTTCGGGC	50.0	50.0	58.1	57.8	1827 bp	1.0	77.9
rps19-rpl23_p5	TATGAACGGCAATGGTGTGA	TGAGTTTGAATTCCGCGAGT	45.0	45.0	57.5	57.8	1905 bp	1.0	75.7