Hotspot

trnM-CAU-atpE

LSC · 50005-50149 · 145 bp

Back to Christella

Pi 0.0207

MarkerSeek score 64.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 3
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCGAGAGTCATTGGTTCGAA	AGGCCAGACTAGAAGCTTCA	50.0	50.0	58.3	58.1	211 bp	1.0	78.4
trnM-CAU-atpE_p2	GCGAGAGTCATTGGTTCGAA	AGAAGCTTCAAATGCTGCCT	50.0	45.0	58.3	58.1	201 bp	1.0	78.2
trnM-CAU-atpE_p3	GCGAGAGTCATTGGTTCGAA	TAAAAGAGCGAAGGCCAGAC	50.0	50.0	58.3	57.9	222 bp	1.0	78.0
trnM-CAU-atpE_p4	GCGAGAGTCATTGGTTCGAA	TTCAGATGGCTCAAGCTGAC	50.0	50.0	58.3	57.9	292 bp	1.0	77.7
trnM-CAU-atpE_p5	AATGGCAGCCTACTTGACTC	AGGCCAGACTAGAAGCTTCA	50.0	50.0	57.6	58.1	257 bp	1.0	77.2