Hotspot

trnS-GGA-trnL-UAA

LSC · 43738-45480 · 1743 bp

Back to Christella

Pi 0.0144
MarkerSeek score 55.40
Primer available yes
Label trnS-GGA-trnL-UAA

Feature Metadata

label_name
trnS-GGA-trnL-UAA
feature_type
igs
region
LSC
length_bp
1743
variable_sites
25
pi
0.0144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-trnL-UAA_p1 TTCGAATCCCTCCCTTTCCT GCGTCTACCATTTCGCCATA 50.0 50.0 58.0 58.1 1786-1788 bp 1.0 79.8
trnS-GGA-trnL-UAA_p2 GGTTCGAATCCCTCCCTTTC GCGTCTACCATTTCGCCATA 55.0 50.0 58.0 58.1 1788-1790 bp 1.0 79.8
trnS-GGA-trnL-UAA_p3 GTTCGAATCCCTCCCTTTCC GCGTCTACCATTTCGCCATA 55.0 50.0 58.0 58.1 1787-1789 bp 1.0 79.8
trnS-GGA-trnL-UAA_p4 GACTTCTAGCTACCGAGGGT GCGTCTACCATTTCGCCATA 55.0 50.0 57.9 58.1 1805-1807 bp 1.0 79.6
trnS-GGA-trnL-UAA_p5 AGACTTCTAGCTACCGAGGG GCGTCTACCATTTCGCCATA 55.0 50.0 57.6 58.1 1806-1808 bp 1.0 77.3