Hotspot

trnS-UGA-psbC

LSC · 31343-31640 · 298 bp

Back to Christella

Pi 0.0346

MarkerSeek score 67.20

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 10
pi: 0.0346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GTCTTGAAAACTGGCATGGC	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.9	57.9	477-479 bp	1.0	82.1
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	TTCTTCTTTTTCGTGGGCCA	55.0	45.0	58.2	57.9	441-443 bp	1.0	81.2
trnS-UGA-psbC_p3	GTCTTGAAAACTGGCATGGC	TCTTCTTTTTCGTGGGCCAT	50.0	45.0	57.9	57.7	476-478 bp	1.0	80.3
trnS-UGA-psbC_p4	GAGATGGTCGAGCGGTTTAA	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.7	57.9	504-506 bp	1.0	80.2
trnS-UGA-psbC_p5	AGATGGTCGAGCGGTTTAAG	TTCTTCTTTTTCGTGGGCCA	50.0	45.0	57.7	57.9	503-505 bp	1.0	80.2