Hotspot

trnG-GCC-psbZ

LSC · 30360-30622 · 263 bp

Back to Christella

Pi 0.0311

MarkerSeek score 67.80

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 263
variable_sites: 8
pi: 0.0311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	TCTGTGGGATAGGCGGATAG	TTGTCTTTTCTGGGGCTTCA	55.0	45.0	58.1	57.3	413-419 bp	1.0	76.1
trnG-GCC-psbZ_p2	ACCTCTTCGTTTTCTGTGGG	TTGTCTTTTCTGGGGCTTCA	50.0	45.0	57.7	57.3	425-431 bp	1.0	74.6
trnG-GCC-psbZ_p3	TCTGTGGGATAGGCGGATAG	TGTCTTTTCTGGGGCTTCAT	55.0	45.0	58.1	57.0	412-418 bp	1.0	74.3
trnG-GCC-psbZ_p4	TCTGTGGGATAGGCGGATAG	TGGGGCTTCATTATGGATTGG	55.0	47.6	58.1	58.0	403-409 bp	1.0	73.8
trnG-GCC-psbZ_p5	TTCTGTGGGATAGGCGGATA	TTGTCTTTTCTGGGGCTTCA	50.0	45.0	57.6	57.3	414-420 bp	1.0	73.6