Hotspot

trnC-GCA-trnG-GCC

LSC · 29842-30288 · 447 bp

Back to Christella

Pi 0.0248

MarkerSeek score 63.30

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 11
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	504-507 bp	1.0	72.1
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	505-508 bp	1.0	71.9
trnC-GCA-trnG-GCC_p3	TTAGGCGACACCCAGATTTG	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	539-542 bp	1.0	71.8
trnC-GCA-trnG-GCC_p4	GTTAGGCGACACCCAGATTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	540-543 bp	1.0	71.8
trnC-GCA-trnG-GCC_p5	TTTCTTGTTAGGCGACACCC	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	546-549 bp	1.0	71.8