Hotspot

ycf2

IRa · 154903-161139 · 6237 bp

Back to Chorispora

Pi 0.00128

MarkerSeek score 53.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6237
variable_sites: 8
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GACGGATAGAGGCGGATTTC	CTCGTCTCAGGTAGTCCCTC	55.0	60.0	57.9	58.3	327-345 bp	1.0	78.5
ycf2_p2	GACGGATAGAGGCGGATTTC	ATCTCGTCTCAGGTAGTCCC	55.0	55.0	57.9	57.6	329-347 bp	1.0	78.3
ycf2_p3	GACGGATAGAGGCGGATTTC	ATCCAACCCATCCTCGAACT	55.0	50.0	57.9	58.4	299-317 bp	1.0	78.0
ycf2_p4	GACGGATAGAGGCGGATTTC	AATCCAACCCATCCTCGAAC	55.0	50.0	57.9	57.6	300-318 bp	1.0	77.9
ycf2_p5	ACGGATAGAGGCGGATTTCT	CTCGTCTCAGGTAGTCCCTC	50.0	60.0	58.3	58.3	326-344 bp	1.0	76.7