Hotspot

ycf1

IRb · 111007-116592 · 5586 bp

Back to Chorispora

Pi 0.00143

MarkerSeek score 49.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5586
variable_sites: 8
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGTAGTACATATCACCAAAA	CGGCTGGAATTTGTCAAACC	31.8	50.0	52.9	57.9	314 bp	1.0	40.8
ycf1_p2	TTGTGTAGTACATATCACCAAA	CGGCTGGAATTTGTCAAACC	31.8	50.0	52.9	57.9	315 bp	1.0	40.8
ycf1_p3	TTGTGTAGTACATATCACCAAAA	CGGCTGGAATTTGTCAAACC	30.4	50.0	53.7	57.9	315 bp	1.0	40.8
ycf1_p4	ATTGTGTAGTACATATCACCAA	CGGCTGGAATTTGTCAAACC	31.8	50.0	52.6	57.9	316 bp	1.0	40.8
ycf1_p5	TGCAGAAATACTATCTATTCTTCCTCA	CGGCTGGAATTTGTCAAACC	33.3	50.0	57.9	57.9	382 bp	1.0	40.6