Hotspot

ndhD

SSC · 124739-126259 · 1521 bp

Back to Chorispora

Pi 0.000657

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 1
pi: 0.000657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGCTCTCACAATACAAAGACC	TGAGTACGCGTTCTTTGGAC	45.5	50.0	58.1	58.2	1617 bp	1.0	61.8
ndhD_p2	GGCTCTCACAATACAAAGACCA	TGAGTACGCGTTCTTTGGAC	45.5	50.0	58.3	58.2	1616 bp	1.0	59.6
ndhD_p3	AGGCTCTCACAATACAAAGACC	TTGAGTACGCGTTCTTTGGA	45.5	45.0	58.1	57.5	1618 bp	1.0	59.5
ndhD_p4	GGCTCTCACAATACAAAGACC	TGAGTACGCGTTCTTTGGAC	47.6	50.0	56.6	58.2	1616 bp	1.0	59.2
ndhD_p5	GGCTCTCACAATACAAAGACCA	TTGAGTACGCGTTCTTTGGA	45.5	45.0	58.3	57.5	1617 bp	1.0	57.3