Hotspot

trnN-AAC-ycf1

IRb · 109837-111006 · 1170 bp

Back to Chorispora

Pi 0.00108

MarkerSeek score 59.60

Primer available yes

Label trnN-AAC-ycf1

Feature Metadata

label_name: trnN-AAC-ycf1
feature_type: igs
region: IRb
length_bp: 1170
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-AAC-ycf1_p1	CCCCAAGTAGGATTCGAACC	ATAGAGTCCGACCACAACGA	55.0	50.0	57.7	58.2	1076-1324 bp	1.0	80.1
trnN-AAC-ycf1_p2	CCCCAAGTAGGATTCGAACC	ACCACAACGACCGAATTGAT	55.0	45.0	57.7	57.8	1066-1314 bp	1.0	80.0
trnN-AAC-ycf1_p3	CCCCAAGTAGGATTCGAACC	GGGACCTATGGATAATGCGG	55.0	55.0	57.7	57.6	1109-1357 bp	1.0	78.2
trnN-AAC-ycf1_p4	CCCCAAGTAGGATTCGAACC	GACCACAACGACCGAATTGA	55.0	50.0	57.7	58.5	1067-1315 bp	1.0	77.5
trnN-AAC-ycf1_p5	CCCCAAGTAGGATTCGAACC	AATAGAGTCCGACCACAACG	55.0	50.0	57.7	57.4	1077-1325 bp	1.0	76.3