Hotspot

rpoB-trnC-UGC

LSC · 25853-26928 · 1076 bp

Back to Chorispora

Pi 0.000929

MarkerSeek score 59.30

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1076
variable_sites: 1
pi: 0.000929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	AGGGGATTTCAAGCCTGTTG	55.0	50.0	57.9	58.1	1196-1197 bp	1.0	78.3
rpoB-trnC-UGC_p2	GTTCCCTCTTTTCCATCCCC	GGGGATTTCAAGCCTGTTGT	55.0	50.0	57.9	58.4	1195-1196 bp	1.0	76.0
rpoB-trnC-UGC_p3	GTTCCCTCTTTTCCATCCCC	GTAGGGGATTTCAAGCCTGT	55.0	50.0	57.9	57.2	1198-1199 bp	1.0	72.4
rpoB-trnC-UGC_p4	TGTTCCCTCTTTTCCATCCC	AGGGGATTTCAAGCCTGTTG	50.0	50.0	57.1	58.1	1197-1198 bp	1.0	72.3
rpoB-trnC-UGC_p5	TCTTTTCCATCCCCGAGCAT	AGGGGATTTCAAGCCTGTTG	50.0	50.0	59.1	58.1	1190-1191 bp	1.0	70.9