Hotspot

ndhA

SSC · 121499-123736 · 2238 bp

Back to Chorilaena

Pi 0.0117

MarkerSeek score 50.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 26
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGCGAATTCAACACTGTTCG	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.0	57.9	2811-2824 bp	1.0	80.0
ndhA_p2	ACCAATGTACCTTGCAGCTC	GCTACGAGCGTCTGGAATAG	50.0	55.0	58.2	58.0	2888-2901 bp	1.0	79.7
ndhA_p3	ACCAATGTACCTTGCAGCTC	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.2	58.0	2589 bp	0.5	58.4
ndhA_p4	GACCAATGTACCTTGCAGCT	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	58.2	58.0	2590 bp	0.5	58.4
ndhA_p5	ACCTTGCAGCTCGTATTGTT	TGTGAGAGTAGAAGCCCCAA	45.0	50.0	57.8	58.0	2581 bp	0.5	58.3