Hotspot

ndhD-psaC

SSC · 118762-118946 · 185 bp

Back to Chorilaena

Pi 0.0337

MarkerSeek score 73.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 185
variable_sites: 6
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGCGGCTATGGGAAAAACTA	GTGTTGGTTGTAAGCGATGC	50.0	50.0	58.3	58.0	308-313 bp	1.0	80.3
ndhD-psaC_p2	TTAAAGAACCCGCGGCTATG	GTGTTGGTTGTAAGCGATGC	50.0	50.0	58.3	58.0	318-323 bp	1.0	80.2
ndhD-psaC_p3	AAAATTAAAGAACCCGCGGC	GTGTTGGTTGTAAGCGATGC	45.0	50.0	57.6	58.0	322-327 bp	1.0	80.1
ndhD-psaC_p4	AATTAAAGAACCCGCGGCTA	GTGTTGGTTGTAAGCGATGC	45.0	50.0	57.6	58.0	320-325 bp	1.0	79.7
ndhD-psaC_p5	AAATTAAAGAACCCGCGGCT	GTGTTGGTTGTAAGCGATGC	45.0	50.0	58.5	58.0	321-326 bp	1.0	79.3