Hotspot

rps3-rpl22

LSC · 84892-85259 · 368 bp

Back to Chorilaena

Pi 0.0387

MarkerSeek score 68.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 12
pi: 0.0387
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TGTTTTGCAAACCAAAGCGA	GTTCTTACGAGGAAGCCCTT	40.0	50.0	57.6	57.2	411-469 bp	1.0	77.1
rps3-rpl22_p2	TGTTTTGCAAACCAAAGCGA	TCAGAGATTCGTGGACGTTC	40.0	50.0	57.6	57.4	485 bp	0.5	52.0
rps3-rpl22_p3	TGTTTTGCAAACCAAAGCGA	CAGAGATTCGTGGACGTTCT	40.0	50.0	57.6	57.4	484 bp	0.5	51.9
rps3-rpl22_p4	TGTTTTGCAAACCAAAGCGA	TGATCAGAGATTCGTGGACG	40.0	50.0	57.6	57.1	488 bp	0.5	50.2
rps3-rpl22_p5	TGTTTTGCAAACCAAAGCGA	GATCAGAGATTCGTGGACGT	40.0	50.0	57.6	57.1	487 bp	0.5	50.2