Hotspot

psbE-petL

LSC · 66059-67490 · 1432 bp

Back to Chorilaena

Pi 0.0102

MarkerSeek score 68.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1432
variable_sites: 14
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.9	56.7	1521-1579 bp	1.0	73.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.9	56.7	1520-1578 bp	1.0	73.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.8	56.7	1444-1502 bp	1.0	72.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.8	56.7	1443-1501 bp	1.0	72.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGCTAGGAGAAAACCAA	45.0	45.0	58.2	56.7	1529-1587 bp	1.0	72.4