Hotspot

trnF-GAA-ndhJ

LSC · 50069-50476 · 408 bp

Back to Chorilaena

Pi 0.0319

MarkerSeek score 58.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 13
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.0	58.0	560 bp	1.0	81.9
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CGTTCCCAATGTGCCTATGA	55.0	50.0	58.0	58.0	802 bp	1.0	81.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCTACGTTCCCAATGTGCC	55.0	50.0	58.0	58.0	807 bp	1.0	81.3
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	959 bp	1.0	81.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	AAAGATGCAGGGTCGTTTGT	50.0	45.0	58.1	58.0	923 bp	1.0	80.9