Hotspot

rps4-trnT-UGU

LSC · 47668-48033 · 366 bp

Back to Chorilaena

Pi 0.0286

MarkerSeek score 68.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 9
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGGCCCTTTACCCGTTAGT	TTTAAGCCCGCTTAGCTCAG	50.0	50.0	57.7	58.0	472-523 bp	1.0	83.2
rps4-trnT-UGU_p2	TAGGCCCTTTACCCGTTAGT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	457-508 bp	1.0	82.4
rps4-trnT-UGU_p3	TAGGCCCTTTACCCGTTAGT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.7	57.8	425-476 bp	1.0	81.7
rps4-trnT-UGU_p4	CCTAGGCCCTTTACCCGTTA	TTTAAGCCCGCTTAGCTCAG	55.0	50.0	58.5	58.0	474-525 bp	1.0	81.2
rps4-trnT-UGU_p5	CCTAGGCCCTTTACCCGTTA	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.5	57.9	459-510 bp	1.0	80.4