Hotspot

psbZ-trnG-GCC

LSC · 37688-38199 · 512 bp

Back to Chorilaena

Pi 0.0373

MarkerSeek score 70.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 19
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.4	58.1	669-671 bp	1.0	79.1
psbZ-trnG-GCC_p2	CGGATGGTTGGTCGAGTAAC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.4	58.1	670-672 bp	1.0	79.1
psbZ-trnG-GCC_p3	TTTGCTTCTCCGGATGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	58.5	58.1	679-681 bp	1.0	78.3
psbZ-trnG-GCC_p4	TTTGCTTCTCCGGATGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	58.5	58.1	680-682 bp	1.0	78.3
psbZ-trnG-GCC_p5	ATTTGCTTCTCCGGATGGTT	AATCGAACCCGCATCTTCTC	45.0	50.0	57.5	58.1	680-682 bp	1.0	77.9