Hotspot

psbC-trnS-UGA

LSC · 36801-37049 · 249 bp

Back to Chorilaena

Pi 0.0407
MarkerSeek score 76.00
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
249
variable_sites
10
pi
0.0407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 GCAGCAGGGTTTGAAAAAGG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.1 58.1 392-395 bp 1.0 81.1
psbC-trnS-UGA_p2 CCTGTTCTTTCGATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.3 58.1 353-356 bp 1.0 80.4
psbC-trnS-UGA_p3 GCAGCAGGGTTTGAAAAAGG GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.1 58.2 338-341 bp 1.0 80.3
psbC-trnS-UGA_p4 CCTGTTCTTTCGATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.3 58.2 299-302 bp 1.0 79.6
psbC-trnS-UGA_p5 TAGGTCATTTATGGCACGCG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.4 58.1 430-433 bp 1.0 78.6