Hotspot

trnC-GCA-petN

LSC · 28881-29501 · 621 bp

Back to Chorilaena

Pi 0.018

MarkerSeek score 63.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 11
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.4	58.1	736-743 bp	1.0	78.6
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CCATACTACGAGCGAAAGGG	50.0	55.0	58.4	57.9	743-750 bp	1.0	78.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CCCATACTACGAGCGAAAGG	50.0	55.0	58.4	57.9	744-751 bp	1.0	78.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CCCCATACTACGAGCGAAAG	50.0	55.0	58.4	57.9	745-752 bp	1.0	78.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	698-705 bp	1.0	78.3