Hotspot

trnK-UUU-rps16

LSC · 4418-4988 · 571 bp

Back to Chorilaena

Pi 0.0233

MarkerSeek score 59.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 13
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	676-681 bp	1.0	83.1
trnK-UUU-rps16_p2	AGCCGTACTTAAAAGCCGAG	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	849-854 bp	1.0	82.3
trnK-UUU-rps16_p3	AGCCGTACTTAAAAGCCGAG	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	850-855 bp	1.0	82.3
trnK-UUU-rps16_p4	AATTGGAACCCTAGGGCTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	924-929 bp	1.0	82.2
trnK-UUU-rps16_p5	CGCGCCAAGAATTCAAACTT	ATTTCAAAGAAAGCGGGGGT	45.0	45.0	57.9	58.0	857-862 bp	1.0	82.0