Hotspot

trnH-GUG-psbA

LSC · 101-478 · 378 bp

Back to Chorilaena

Pi 0.0372

MarkerSeek score 62.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 14
pi: 0.0372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ATTTCAGGCAGAGCACAACA	55.0	45.0	58.1	58.0	906-908 bp	1.0	80.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TTTCAGGCAGAGCACAACAT	55.0	45.0	58.1	58.0	905-907 bp	1.0	80.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	ATTTCAGGCAGAGCACAACA	50.0	45.0	57.9	58.0	920-922 bp	1.0	80.4
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TTTCAGGCAGAGCACAACAT	50.0	45.0	57.9	58.0	919-921 bp	1.0	80.4
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	TTTCAGGCAGAGCACAACAT	50.0	45.0	57.9	58.0	918-920 bp	1.0	80.4