Hotspot

rps2

Genome · 38784-39479 · 696 bp

Back to Chorda

Pi 0.415
MarkerSeek score 80.70
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
Genome
length_bp
696
variable_sites
284
pi
0.415
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 GCTGCTAAGCCTTTTTGACG ACACGAGCTGCTATTCAAGG 50.0 50.0 58.0 58.0 1278 bp 0.5 59.6
rps2_p2 GCTGCTAAGCCTTTTTGACG CACGAGCTGCTATTCAAGGT 50.0 50.0 58.0 58.0 1277 bp 0.5 59.6
rps2_p3 TTCTGCCTCAATCGCCTTTT ACACGAGCTGCTATTCAAGG 45.0 50.0 58.1 58.0 1525 bp 0.5 59.2
rps2_p4 TTCTGCCTCAATCGCCTTTT CACGAGCTGCTATTCAAGGT 45.0 50.0 58.1 58.0 1524 bp 0.5 59.2
rps2_p5 TTTCTGCCTCAATCGCCTTT CACGAGCTGCTATTCAAGGT 45.0 50.0 58.1 58.0 1525 bp 0.5 59.2