Hotspot

psbC

Genome · 119342-120766 · 1425 bp

Back to Chorda

Pi 0.46
MarkerSeek score 79.20
Primer available yes
Label psbC

Feature Metadata

label_name
psbC
feature_type
gene
region
Genome
length_bp
1425
variable_sites
653
pi
0.46
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC_p1 AAAACTCAACGCGTACCAGT GCAAACCGTTTTTGGTCACA 45.0 45.0 58.1 58.0 2374 bp 0.5 59.5
psbC_p2 AACTCAACGCGTACCAGTAC GCAAACCGTTTTTGGTCACA 50.0 45.0 57.9 58.0 2372 bp 0.5 59.5
psbC_p3 AAAACTCAACGCGTACCAGT TTGGTATTGTTGGGCTTGCT 45.0 45.0 58.1 58.0 2268 bp 0.5 59.5
psbC_p4 AACTCAACGCGTACCAGTAC TTGGTATTGTTGGGCTTGCT 50.0 45.0 57.9 58.0 2266 bp 0.5 59.5
psbC_p5 AAAACTCAACGCGTACCAGT CAGGTCTTTGGACAAGTGCT 45.0 50.0 58.1 58.0 2289 bp 0.5 59.3