Hotspot

psbB

Genome · 4354-5886 · 1533 bp

Back to Chorda

Pi 0.381
MarkerSeek score 66.00
Primer available yes
Label psbB

Feature Metadata

label_name
psbB
feature_type
gene
region
Genome
length_bp
1533
variable_sites
104
pi
0.381
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.18
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB_p1 CTTGAAGACGCTGGTGCTAT CCTATACAGCGTTTGGACCA 50.0 50.0 58.0 57.3 2509 bp 0.5 54.4
psbB_p2 CTTGAAGACGCTGGTGCTAT ACCTATACAGCGTTTGGACC 50.0 50.0 58.0 57.3 2510 bp 0.5 54.4
psbB_p3 AAACACTTGAAGACGCTGGT CCTATACAGCGTTTGGACCA 45.0 50.0 58.0 57.3 2514 bp 0.5 54.2
psbB_p4 AAACACTTGAAGACGCTGGT ACCTATACAGCGTTTGGACC 45.0 50.0 58.0 57.3 2515 bp 0.5 54.2
psbB_p5 CTCCTGCACGGCAAGAATAT CCTATACAGCGTTTGGACCA 50.0 50.0 58.0 57.3 2149 bp 0.5 54.2