Hotspot

dnaB

Genome · 1-1746 · 1746 bp

Back to Chorda

Pi 0.438
MarkerSeek score 69.50
Primer available yes
Label dnaB

Feature Metadata

label_name
dnaB
feature_type
gene
region
Genome
length_bp
1746
variable_sites
231
pi
0.438
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.30
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB_p1 GATTGGTGGGGCAAACCTTA GTTTTGCCCATTGAAGGACG 50.0 50.0 58.1 57.9 402 bp 0.5 58.9
dnaB_p2 TGGTGGGGCAAACCTTATTC GTTTTGCCCATTGAAGGACG 50.0 50.0 58.1 57.9 399 bp 0.5 58.9
dnaB_p3 GATTGGTGGGGCAAACCTTA GCAGTTTTGCCCATTGAAGG 50.0 50.0 58.1 58.2 405 bp 0.5 57.8
dnaB_p4 TGGTGGGGCAAACCTTATTC GCAGTTTTGCCCATTGAAGG 50.0 50.0 58.1 58.2 402 bp 0.5 57.8
dnaB_p5 GGTGGGGCAAACCTTATTCT GTTTTGCCCATTGAAGGACG 50.0 50.0 57.8 57.9 398 bp 0.5 57.6