Hotspot

clpC

Genome · 77814-80492 · 2679 bp

Pi 0.405

MarkerSeek score 67.10

Primer available yes

Label clpC

Feature Metadata

label_name: clpC
feature_type: gene
region: Genome
length_bp: 2679
variable_sites: 266
pi: 0.405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.25
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC_p1	TGCCAAGTTAGGAGGTACGT	AGTCCCAGGTGACCCAATTA	50.0	50.0	58.4	57.9	2858 bp	0.5	56.7
clpC_p2	TGCCAAGTTAGGAGGTACGT	AAGTAGAAGCAGTGGGTTGC	50.0	50.0	58.4	58.1	2927 bp	0.5	56.3
clpC_p3	TGCCAAGTTAGGAGGTACGT	GCAGTGGGTTGCTAACTCAA	50.0	50.0	58.4	58.4	2919 bp	0.5	53.9
clpC_p4	GCCAAGTTAGGAGGTACGTT	AGTCCCAGGTGACCCAATTA	50.0	50.0	57.2	57.9	2857 bp	0.5	53.5
clpC_p5	GCCAAGTTAGGAGGTACGTT	AAGTAGAAGCAGTGGGTTGC	50.0	50.0	57.2	58.1	2926 bp	0.5	53.1