Hotspot

atpF

Genome · 35762-36286 · 525 bp

Pi 0.414

MarkerSeek score 79.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 525
variable_sites: 190
pi: 0.414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGCCTCTGAATATGGATTGGC	GCTTAGTTGTGGCCTTAGCT	47.6	50.0	57.8	57.9	1260 bp	0.5	49.9
atpF_p2	GGATTGGCGATTTTTGAACCA	GCTTAGTTGTGGCCTTAGCT	42.9	50.0	57.7	57.9	1247 bp	0.5	48.6
atpF_p3	TGGATTGGCGATTTTTGAACC	GCTTAGTTGTGGCCTTAGCT	42.9	50.0	57.7	57.9	1248 bp	0.5	48.6
atpF_p4	CCTCTGAATATGGATTGGCGA	GCTTAGTTGTGGCCTTAGCT	47.6	50.0	57.6	57.9	1258 bp	0.5	47.8
atpF_p5	AGCCTCTGAATATGGATTGGC	TTTACGGCTTAGTTGTGGCC	47.6	50.0	57.8	58.5	1266 bp	0.5	47.0