Hotspot

ccsA-psbK

Genome · 89668-90085 · 418 bp

Back to Chorda

Pi 0.458
MarkerSeek score 71.60
Primer available yes
Label ccsA-psbK

Feature Metadata

label_name
ccsA-psbK
feature_type
igs
region
Genome
length_bp
418
variable_sites
97
pi
0.458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.51
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psbK_p1 GGCTTACATAGTTATGGCTGGT CGAAGCTTGCCAAAGGAAGG 45.5 55.0 57.9 59.8 568 bp 0.5 29.2
ccsA-psbK_p2 GGCTTACATAGTTATGGCTGGT CGAAGCTTGCCAAAGGAAG 45.5 52.6 57.9 57.2 568 bp 0.5 29.1
ccsA-psbK_p3 AGGCTTACATAGTTATGGCTGG CGAAGCTTGCCAAAGGAAGG 45.5 55.0 57.6 59.8 569 bp 0.5 27.1
ccsA-psbK_p4 AGGCTTACATAGTTATGGCTGG CGAAGCTTGCCAAAGGAAG 45.5 52.6 57.6 57.2 569 bp 0.5 26.9
ccsA-psbK_p5 GGCTTACATAGTTATGGCTGGT CCAAAGGAAGGCAAGTAACA 45.5 45.0 57.9 55.9 559 bp 0.5 26.1