Hotspot

rpoC1

LSC · 20981-23782 · 2802 bp

Back to Chonemorpha

Pi 0.00214

MarkerSeek score 57.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2802
variable_sites: 6
pi: 0.00214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AATCCCGGAGAGACCTGAAT	ATGCGCCAGAAATTTGCTTC	50.0	45.0	57.8	58.0	2875-2876 bp	1.0	78.7
rpoC1_p2	ATCCCGGAGAGACCTGAATT	ATGCGCCAGAAATTTGCTTC	50.0	45.0	57.8	58.0	2874-2875 bp	1.0	78.7
rpoC1_p3	AGACCTGAATTCCTACCGGT	ATGCGCCAGAAATTTGCTTC	50.0	45.0	57.8	58.0	2865-2866 bp	1.0	78.2
rpoC1_p4	GGTTTGGGTCAGTGATTGGA	ATGCGCCAGAAATTTGCTTC	50.0	45.0	57.7	58.0	2942-2943 bp	1.0	77.9
rpoC1_p5	CGCGTAGAAATTCCGGAACT	ATGCGCCAGAAATTTGCTTC	50.0	45.0	58.4	58.0	2897-2898 bp	1.0	77.3