Hotspot

ccsA

SSC · 115971-116939 · 969 bp

Back to Chonemorpha

Pi 0.00103

MarkerSeek score 50.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATGTCATCTTCTAA	ACTGTAATGTGGGTGTGCTG	50.0	50.0	58.0	58.1	1098 bp	1.0	78.9
ccsA_p2	GGCGGCATGTCATCTTCTAA	CTGTAATGTGGGTGTGCTGT	50.0	50.0	58.0	58.1	1097 bp	1.0	78.9
ccsA_p3	GGCGGCATGTCATCTTCTAA	GTTCGTGCCCATTCACCTAA	50.0	50.0	58.0	58.2	1132 bp	1.0	78.2
ccsA_p4	GGCGGCATGTCATCTTCTAA	TAATGTGGGTGTGCTGTGTT	50.0	45.0	58.0	57.6	1094 bp	1.0	76.8
ccsA_p5	GGCGGCATGTCATCTTCTAA	GTAATGTGGGTGTGCTGTGT	50.0	50.0	58.0	58.4	1095 bp	1.0	76.5