Hotspot

ccsA-ndhD

SSC · 116940-117187 · 248 bp

Back to Chonemorpha

Pi 0.0363

MarkerSeek score 67.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 248
variable_sites: 9
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCGGCAATTGTAGCGTCT	GGTTGAGAAGGTTGAAGCGA	45.0	50.0	58.0	58.1	410 bp	1.0	80.6
ccsA-ndhD_p2	ATTCGGCAATTGTAGCGTCT	TCGGTTGAGAAGGTTGAAGC	45.0	50.0	58.0	58.1	412 bp	1.0	80.6
ccsA-ndhD_p3	ATTCGGCAATTGTAGCGTCT	TGAGAAGGTTGAAGCGATCC	45.0	50.0	58.0	57.6	407 bp	1.0	78.5
ccsA-ndhD_p4	ATTCGGCAATTGTAGCGTCT	ACCCCGATTTCGTTCTTTCA	45.0	45.0	58.0	57.4	435 bp	1.0	77.0
ccsA-ndhD_p5	ATTCGGCAATTGTAGCGTCT	GAGAAGGTTGAAGCGATCCT	45.0	50.0	58.0	57.3	406 bp	1.0	76.1