Hotspot

ndhF-rpl32

SSC · 113954-114733 · 780 bp

Back to Chonemorpha

Pi 0.00655

MarkerSeek score 54.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 780
variable_sites: 5
pi: 0.00655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	58.0	57.3	922-939 bp	1.0	44.1
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TCCAAATATTTTTCCGAATACGCT	45.0	33.3	58.0	57.3	920-937 bp	1.0	44.0
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TCCAAATATTTTTCCGAATACGCT	45.0	33.3	58.0	57.3	921-938 bp	1.0	44.0
ndhF-rpl32_p4	TCGGAAAAAGAAGAAGCCCC	TCCAAATATTTTTCCGAATACGCT	50.0	33.3	58.1	57.3	915-932 bp	1.0	43.7
ndhF-rpl32_p5	GGTATGAGCCACCCGTATTG	TCCAAATATTTTTCCGAATACGCT	55.0	33.3	58.1	57.3	859-876 bp	1.0	43.6