Hotspot

atpH-atpI

LSC · 13563-14782 · 1220 bp

Back to Chonemorpha

Pi 0.00496

MarkerSeek score 62.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1220
variable_sites: 6
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	1305-1307 bp	1.0	79.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CCGCAGCTTATATCGGTGAA	45.0	50.0	58.0	57.9	1291-1293 bp	1.0	79.6
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	1388-1390 bp	1.0	78.8
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	57.9	57.9	1392-1394 bp	1.0	78.8
atpH-atpI_p5	TACCCTCTACAGCTTGACCC	CCGCAGCTTATATCGGTGAA	55.0	50.0	58.1	57.9	1374-1376 bp	1.0	78.7