Hotspot

trnS-trnG

LSC · 8414-9066 · 653 bp

Back to Chonemorpha

Pi 0.00332

MarkerSeek score 64.70

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 2
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	ATTAGCAATCCGCCGCTTTA	TCAAACCGAAAACAAAGACCC	45.0	42.9	58.0	57.2	723-772 bp	1.0	69.6
trnS-trnG_p2	ATTAGCAATCCGCCGCTTTA	CAAACCGAAAACAAAGACCCT	45.0	42.9	58.0	57.2	722-771 bp	1.0	69.6
trnS-trnG_p3	TCAAGTCATCAAGGGAAGCG	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	789-838 bp	1.0	69.1
trnS-trnG_p4	TCAAGTCATCAAGGGAAGCG	CAAACCGAAAACAAAGACCCT	50.0	42.9	57.9	57.2	788-837 bp	1.0	69.1
trnS-trnG_p5	CATCAAGGGAAGCGGAAAGA	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	783-832 bp	1.0	69.0