Hotspot

rps16-trnQ

LSC · 6083-7367 · 1285 bp

Back to Chonemorpha

Pi 0.00713

MarkerSeek score 60.00

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 9
pi: 0.00713
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1339-1362 bp	1.0	75.4
rps16-trnQ_p2	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1365-1388 bp	1.0	75.3
rps16-trnQ_p3	TGTACTTCAAGTCGCACGTT	TTCGGAGGTTCGAATCCTTC	45.0	50.0	57.8	57.3	1347-1370 bp	1.0	74.8
rps16-trnQ_p4	TGTACTTCAAGTCGCACGTT	TAAGGCAACGGGTTTTGGTC	45.0	50.0	57.8	58.7	1373-1396 bp	1.0	74.7
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	AAATCTAAATGGGGCGTGGC	45.0	50.0	58.1	58.9	1392-1415 bp	1.0	73.6