Hotspot

trnK-rps16

LSC · 4206-4968 · 763 bp

Back to Chonemorpha

Pi 0.00656

MarkerSeek score 60.00

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 5
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CCGATGGTATGGACGAATCC	GGGCTCAACCTACAGGAACT	55.0	55.0	57.9	59.0	947 bp	1.0	71.7
trnK-rps16_p2	AAAGCCGAGTACTCTACCGT	GGGCTCAACCTACAGGAACT	50.0	55.0	58.2	59.0	886 bp	1.0	71.1
trnK-rps16_p3	AAGCCGAGTACTCTACCGTT	GGGCTCAACCTACAGGAACT	50.0	55.0	58.2	59.0	885 bp	1.0	71.1
trnK-rps16_p4	CGTAAAAGATCCTAGCCGCA	GGGCTCAACCTACAGGAACT	50.0	55.0	57.8	59.0	910 bp	1.0	70.7
trnK-rps16_p5	TGCGTAAAAGATCCTAGCCG	GGGCTCAACCTACAGGAACT	50.0	55.0	57.8	59.0	912 bp	1.0	70.7