Hotspot

matK-trnK

LSC · 3470-4168 · 699 bp

Back to Chonemorpha

Pi 0.00286

MarkerSeek score 60.40

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 2
pi: 0.00286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	ACTATGGCCATGAGCAAGTG	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	831 bp	1.0	78.5
matK-trnK_p2	ACTATGGCCATGAGCAAGTG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	830 bp	1.0	78.5
matK-trnK_p3	ACTATGGCCATGAGCAAGTG	GGGTTGCTAACTCAACGGTA	50.0	50.0	58.0	57.5	844 bp	1.0	76.1
matK-trnK_p4	ACTATGGCCATGAGCAAGTG	TTCGGGTTGCTAACTCAACG	50.0	50.0	58.0	58.5	847 bp	1.0	75.8
matK-trnK_p5	ACTATGGCCATGAGCAAGTG	CAACGGTAGAGTACTCGGCT	50.0	55.0	58.0	58.9	832 bp	1.0	72.6