Hotspot

ycf37

Genome · 25941-26453 · 513 bp

Back to Chondracanthus

Pi 0.105

MarkerSeek score 77.10

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 513
variable_sites: 54
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	GAGCTTACGGGATATAGCGC	AAAATGGCGCGATGACAGTA	55.0	45.0	58.1	58.3	936-959 bp	1.0	83.8
ycf37_p2	GAGCTTACGGGATATAGCGC	AAATGGCGCGATGACAGTAA	55.0	45.0	58.1	58.3	935-958 bp	1.0	83.8
ycf37_p3	GAGCTTACGGGATATAGCGC	AATGGCGCGATGACAGTAAA	55.0	45.0	58.1	58.3	934-957 bp	1.0	83.8
ycf37_p4	GAGCTTACGGGATATAGCGC	ATGGCGCGATGACAGTAAAA	55.0	45.0	58.1	58.3	933-956 bp	1.0	83.8
ycf37_p5	GCTTACGGGATATAGCGCAG	ATGGCGCGATGACAGTAAAA	55.0	45.0	58.4	58.3	931-954 bp	1.0	81.6