Hotspot

ycf35

Genome · 9824-10210 · 387 bp

Back to Chondracanthus

Pi 0.0904

MarkerSeek score 73.30

Primer available yes

Label ycf35

Feature Metadata

label_name: ycf35
feature_type: gene
region: Genome
length_bp: 387
variable_sites: 35
pi: 0.0904
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35_p1	GTGGAATTGGTAGACACGCT	AAGATGCAGCTTCCCAAACA	50.0	45.0	57.9	58.0	1037-1043 bp	1.0	84.0
ycf35_p2	CCCCAGGAAGGATTTGAACC	AAGATGCAGCTTCCCAAACA	55.0	45.0	58.1	58.0	1253-1259 bp	1.0	84.0
ycf35_p3	CTTAACCTCCCCAGGAAGGA	AAGATGCAGCTTCCCAAACA	55.0	45.0	58.0	58.0	1267 bp	0.5	59.7
ycf35_p4	CTTAACCTCCCCAGGAAGGA	CTGCAACTGGTTCACGGATA	55.0	50.0	58.0	57.9	1168 bp	0.5	59.0
ycf35_p5	AACCTTAACCTCCCCAGGAA	AAGATGCAGCTTCCCAAACA	50.0	45.0	57.9	58.0	1270 bp	0.5	58.9