Hotspot

syfB

Genome · 163110-165275 · 2166 bp

Back to Chondracanthus

Pi 0.07

MarkerSeek score 76.50

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2166
variable_sites: 151
pi: 0.07
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	TTGAGGAAAGTCCGGTCTCT	TGCAAACGACAATGAGCTATC	50.0	42.9	58.0	57.0	2607 bp	0.5	43.8
syfB_p2	TTGAGGAAAGTCCGGTCTCT	AGGTGAGATAACCGGAATGT	50.0	45.0	58.0	55.9	2769 bp	0.5	43.4
syfB_p3	TTACGAGGAAAGTGCCACAG	TGCAAACGACAATGAGCTATC	50.0	42.9	57.8	57.0	2548 bp	0.5	42.6
syfB_p4	TTACGAGGAAAGTGCCACAG	AGGTGAGATAACCGGAATGT	50.0	45.0	57.8	55.9	2710 bp	0.5	42.1
syfB_p5	TTGAGGAAAGTCCGGTCTCT	TGCAAACGACAATGAGCTAT	50.0	40.0	58.0	55.8	2607 bp	0.5	42.0