Hotspot

infB

Genome · 93572-95893 · 2322 bp

Back to Chondracanthus

Pi 0.0746

MarkerSeek score 76.00

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2322
variable_sites: 172
pi: 0.0746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	GCGTTCTACTGGCCTTAGTT	ACGTGCTCTCCATTACTTGC	50.0	50.0	57.6	58.3	2919-2936 bp	1.0	80.8
infB_p2	GCGTTCTACTGGCCTTAGTT	CGTGCTCTCCATTACTTGCA	50.0	50.0	57.6	58.3	2918-2935 bp	1.0	80.8
infB_p3	GCGTTCTACTGGCCTTAGTT	GCTTTCACGTGCTCTCCATT	50.0	50.0	57.6	58.8	2925-2942 bp	1.0	76.3
infB_p4	GCGTTCTACTGGCCTTAGTT	TCACGTGCTCTCCATTACTTG	50.0	47.6	57.6	58.0	2938 bp	0.5	48.6
infB_p5	GCGTTCTACTGGCCTTAGTT	CACCTTCTGATTGAGCTGACA	50.0	47.6	57.6	58.0	2818 bp	0.5	48.5