Hotspot

psb28-trnQ(ttg)

Genome · 165682-166067 · 386 bp

Back to Chondracanthus

Pi 0.116

MarkerSeek score 81.20

Primer available yes

Label psb28-trnQ(ttg)

Feature Metadata

label_name: psb28-trnQ(ttg)
feature_type: igs
region: Genome
length_bp: 386
variable_sites: 42
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psb28-trnQ(ttg)_p1	ACCTTTAATAAATTGAATACTTGCCA	GAATCCTCCCACCCCAGATA	26.9	55.0	55.9	57.9	412-433 bp	1.0	52.0
psb28-trnQ(ttg)_p2	ACCTTTAATAAATTGAATACTTGCCA	TAGGTTCGAATCCTCCCACC	26.9	55.0	55.9	58.5	419-440 bp	1.0	51.8
psb28-trnQ(ttg)_p3	ACCTTTAATAAATTGAATACTTGCCA	CTTTGACTCCGCCATTCGTA	26.9	50.0	55.9	58.0	437-458 bp	1.0	51.5
psb28-trnQ(ttg)_p4	ACCTTTAATAAATTGAATACTTGCCA	TTTGACTCCGCCATTCGTAG	26.9	50.0	55.9	58.0	436-457 bp	1.0	51.5
psb28-trnQ(ttg)_p5	ACCTTTAATAAATTGAATACTTGCCA	CATATGGGGTGTAGCCAAGC	26.9	55.0	55.9	58.4	471-492 bp	1.0	50.8