Hotspot

trnD(gtc)-trnS(gct)

Genome · 145980-146169 · 190 bp

Back to Chondracanthus

Pi 0.109

MarkerSeek score 76.80

Primer available yes

Label trnD(gtc)-trnS(gct)

Feature Metadata

label_name: trnD(gtc)-trnS(gct)
feature_type: igs
region: Genome
length_bp: 190
variable_sites: 16
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD(gtc)-trnS(gct)_p1	CGGTGCTCTAACCAGTTGAA	GTGGCTGAGAGGTCTAAAGC	50.0	55.0	57.8	58.0	262-305 bp	1.0	87.1
trnD(gtc)-trnS(gct)_p2	CGGTGCTCTAACCAGTTGAA	AGGTCTAAAGCGGCAGATTG	50.0	50.0	57.8	58.0	253-296 bp	1.0	87.1
trnD(gtc)-trnS(gct)_p3	CGGTGCTCTAACCAGTTGAA	GAGGTGGCTGAGAGGTCTAA	50.0	55.0	57.8	58.1	265-308 bp	1.0	86.0
trnD(gtc)-trnS(gct)_p4	TCAAGTAAACGGGACTGACG	AGGTCTAAAGCGGCAGATTG	50.0	50.0	57.6	58.0	306-349 bp	1.0	84.3
trnD(gtc)-trnS(gct)_p5	TCAAGTAAACGGGACTGACG	GTGGCTGAGAGGTCTAAAGC	50.0	55.0	57.6	58.0	315-358 bp	1.0	84.2