Hotspot

trnL(caa)-sufB

Genome · 73940-74887 · 948 bp

Back to Chondracanthus

Pi 0.0728
MarkerSeek score 76.70
Primer available yes
Label trnL(caa)-sufB

Feature Metadata

label_name
trnL(caa)-sufB
feature_type
igs
region
Genome
length_bp
948
variable_sites
69
pi
0.0728
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(caa)-sufB_p1 TGCGTATACCAATTTCGCCA CTCCCATAGGAACAACAGATCC 45.0 50.0 58.0 57.9 1534 bp 0.5 43.2
trnL(caa)-sufB_p2 ACCAATTTCGCCATCTACCC CTCCCATAGGAACAACAGATCC 50.0 50.0 57.9 57.9 1527 bp 0.5 42.9
trnL(caa)-sufB_p3 TGCGTATACCAATTTCGCCA TCCCATAGGAACAACAGATCC 45.0 47.6 58.0 56.8 1533 bp 0.5 42.1
trnL(caa)-sufB_p4 TTCGTACAATTTGACGCCCT CTCCCATAGGAACAACAGATCC 45.0 50.0 57.8 57.9 1674 bp 0.5 42.0
trnL(caa)-sufB_p5 ACCAATTTCGCCATCTACCC TCCCATAGGAACAACAGATCC 50.0 47.6 57.9 56.8 1526 bp 0.5 41.9