Hotspot

cemA-ycf58

Genome · 69414-71768 · 2355 bp

Back to Chondracanthus

Pi 0.0956

MarkerSeek score 78.50

Primer available yes

Label cemA-ycf58

Feature Metadata

label_name: cemA-ycf58
feature_type: igs
region: Genome
length_bp: 2355
variable_sites: 222
pi: 0.0956
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf58_p1	AACCGCTGAAGAGCTTTCAT	GGAAAGCCAACCACCTTCTA	45.0	50.0	57.8	57.4	2877 bp	0.5	53.8
cemA-ycf58_p2	ACCGCTGAAGAGCTTTCATT	GGAAAGCCAACCACCTTCTA	45.0	50.0	57.8	57.4	2876 bp	0.5	53.8
cemA-ycf58_p3	AACCGCTGAAGAGCTTTCAT	GGAAAGCCAACCACCTTCTAT	45.0	47.6	57.8	57.9	2877 bp	0.5	49.8
cemA-ycf58_p4	ACCGCTGAAGAGCTTTCATT	GGAAAGCCAACCACCTTCTAT	45.0	47.6	57.8	57.9	2876 bp	0.5	49.8
cemA-ycf58_p5	TAACCGCTGAAGAGCTTTCA	GGAAAGCCAACCACCTTCTA	45.0	50.0	57.2	57.4	2878 bp	0.5	48.7