Hotspot

trnG(tcc)-trnMe(cat)

Genome · 66387-66412 · 26 bp

Back to Chondracanthus

Pi 0.192

MarkerSeek score 77.50

Primer available yes

Label trnG(tcc)-trnMe(cat)

Feature Metadata

label_name: trnG(tcc)-trnMe(cat)
feature_type: igs
region: Genome
length_bp: 26
variable_sites: 5
pi: 0.192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(tcc)-trnMe(cat)_p1	TTCCAAGCTAATGATGCGGG	CCATCCTGTAAAGGACGTCG	50.0	55.0	58.3	58.1	114-122 bp	1.0	80.2
trnG(tcc)-trnMe(cat)_p2	GCTAATGATGCGGGTTCGAT	CCATCCTGTAAAGGACGTCG	50.0	55.0	58.5	58.1	108-116 bp	1.0	79.1
trnG(tcc)-trnMe(cat)_p3	TTCCAAGCTAATGATGCGGG	GCCATCCTGTAAAGGACGTC	50.0	55.0	58.3	58.3	115-123 bp	1.0	78.0
trnG(tcc)-trnMe(cat)_p4	CCAAGCTAATGATGCGGGTT	CCATCCTGTAAAGGACGTCG	50.0	55.0	58.6	58.1	112-120 bp	1.0	78.0
trnG(tcc)-trnMe(cat)_p5	TTCCAAGCTAATGATGCGGG	AATCCGCCATCCTGTAAAGG	50.0	50.0	58.3	57.7	120-128 bp	1.0	77.9