Hotspot

trnL(taa)-trnC(gca)

Genome · 60421-60439 · 19 bp

Back to Chondracanthus

Pi 0.105

MarkerSeek score 78.60

Primer available yes

Label trnL(taa)-trnC(gca)

Feature Metadata

label_name: trnL(taa)-trnC(gca)
feature_type: igs
region: Genome
length_bp: 19
variable_sites: 2
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(taa)-trnC(gca)_p1	GTCTACCATTCCACCACACC	AGGCGGTATAGCTAAGTGGT	55.0	50.0	57.9	57.9	117 bp	1.0	79.8
trnL(taa)-trnC(gca)_p2	GTCTACCATTCCACCACACC	GGTAAGGCAGAGGATTGCAA	55.0	50.0	57.9	57.9	100 bp	1.0	79.7
trnL(taa)-trnC(gca)_p3	GTCTACCATTCCACCACACC	AGGCAGAGGATTGCAAATCC	55.0	50.0	57.9	58.2	96 bp	1.0	79.0
trnL(taa)-trnC(gca)_p4	GTCTACCATTCCACCACACC	GCTAAGTGGTAAGGCAGAGG	55.0	55.0	57.9	57.7	107 bp	1.0	78.1
trnL(taa)-trnC(gca)_p5	GGGATTTGAACCCTCACGAT	AGGCGGTATAGCTAAGTGGT	50.0	50.0	57.6	57.9	169 bp	1.0	76.9