Hotspot

rpl32-psbY

Genome · 14777-14946 · 170 bp

Back to Chondracanthus

Pi 0.127

MarkerSeek score 76.80

Primer available yes

Label rpl32-psbY

Feature Metadata

label_name: rpl32-psbY
feature_type: igs
region: Genome
length_bp: 170
variable_sites: 19
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-psbY_p1	GCCTTTACGTTTCCATTGAGC	TAGACTAATTGCTGGCTGCG	47.6	50.0	58.1	58.1	694-714 bp	1.0	77.6
rpl32-psbY_p2	GCCTTTACGTTTCCATTGAGC	CTAATTGCTGGCTGCGAAAC	47.6	50.0	58.1	58.1	690-710 bp	1.0	77.3
rpl32-psbY_p3	GCCTTTACGTTTCCATTGAGC	GCACGACATGTCTCTTCACA	47.6	50.0	58.1	58.2	834-854 bp	1.0	75.6
rpl32-psbY_p4	GCCTTTACGTTTCCATTGAGC	AACTCCTGTATTGGCAGCAG	47.6	50.0	58.1	57.9	321 bp	0.5	50.0
rpl32-psbY_p5	CCTGAGGGGCAATAAATGTGT	TAGACTAATTGCTGGCTGCG	47.6	50.0	58.3	58.1	1132 bp	0.5	49.4