Hotspot

trnN(gtt)-trnL(tag)

Genome · 9588-9724 · 137 bp

Back to Chondracanthus

Pi 0.139
MarkerSeek score 77.50
Primer available yes
Label trnN(gtt)-trnL(tag)

Feature Metadata

label_name
trnN(gtt)-trnL(tag)
feature_type
igs
region
Genome
length_bp
137
variable_sites
19
pi
0.139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN(gtt)-trnL(tag)_p1 CTACCACTGAGCTACTGAGGA AGCGTGTCTACCAATTCCAC 52.4 50.0 58.0 57.9 187 bp 1.0 79.2
trnN(gtt)-trnL(tag)_p2 TCTACCACTGAGCTACTGAGG AGCGTGTCTACCAATTCCAC 52.4 50.0 58.0 57.9 188 bp 1.0 79.2
trnN(gtt)-trnL(tag)_p3 CTACCACTGAGCTACTGAGGA CTACCAATTCCACCACGTCC 52.4 55.0 58.0 58.3 180 bp 1.0 77.8
trnN(gtt)-trnL(tag)_p4 TCTACCACTGAGCTACTGAGG CTACCAATTCCACCACGTCC 52.4 55.0 58.0 58.3 181 bp 1.0 77.8
trnN(gtt)-trnL(tag)_p5 ACCAATCGGTTAACAGCCG AGCGTGTCTACCAATTCCAC 52.6 50.0 58.2 57.9 212 bp 1.0 77.2